Target Protein Production
No structure without protein. No screening without pure material. We deliver AI-optimized expression across bacterial, yeast, insect, and mammalian systems — validated through AI-Assisted X-ray Crystallography and Cryo-EM.
Creative Biostructure at a Glance
Over a decade of trusted expertise powering biotech, pharma, and research institutions worldwide to advance therapeutic innovation.
Why Partner With Us
Most structural biology programs fail not because the target is intractable — but because protein production is fragmented. Virtual biotechs burn runway coordinating expression shops, purification facilities, and quality labs. Pharma teams lose months to batch inconsistencies that crash crystallization trials. We built this platform to eliminate that friction: one team where AI-driven expression optimization, multi-system fermentation, and crystal-grade purification share the same milestone clock.
Your CapEx is in compute and chemistry. Ours is in fermentation infrastructure and purification expertise.
| Stage | What We Deliver | What You Don't Need to Build |
|---|---|---|
| AI Expression Design | AlphaFold-guided construct design, host prediction, codon optimization | Molecular biology team |
| Multi-System Expression | Parallel screening across E. coli, yeast, insect, mammalian with ML-optimized conditions | Fermentation suite |
| Crystal-Grade Purification | IMAC, IEX, SEC with real-time NanoDSF/DLS/SEC-MALS quality gates | Chromatography infrastructure |
| Data Handoff | Structural readiness report + direct transition to Crystallography or Cryo-EM | — |
Production-Ready Deliverables: Every protein ships with a quality certificate (Tm, PDI, oligomeric state), structural readiness assessment, and direct handoff to downstream structural biology services.
- ✓ Milestone-based pricing aligned with your fundraising cycles
- ✓ No vendor coordination overhead — expression, purification, and validation under a single project manager
Membrane proteins. Large complexes. Glycoproteins. "Undruggable" is our starting point.
Proven track record where others fail
GPCRs, ion channels, multi-subunit complexes, and nucleic acid-protein assemblies — targets that crash standard expression pipelines.
Multi-modal pivot capability
When E. coli yields inclusion bodies, we pivot to mammalian expression with nanodisc assembly; when yeast underglycosylates, we deploy baculovirus with AI-optimized MOI.
IP firewall & encrypted data infrastructure
Full audit trails, GLP-ready documentation, client retains 100% ownership of all sequences and expression data.
Core Service Modules
Service Module At-a-Glance
| Service | Core Capability | Structural + Computational Integration | Typical Timeline |
|---|---|---|---|
| Gene-to-Protein Production | AI host prediction, codon optimization, parallel expression screening | AlphaFold-guided construct boundaries; Homology Modeling for complex stoichiometry | 2–4 weeks |
| Crystal Grade Protein Preparation | Multi-step chromatography, real-time QC, structural readiness validation | NanoDSF/DLS/SEC-MALS quality gates; direct handoff to Crystallography or Cryo-EM | 2–4 weeks |
Gene-to-Protein Production
AI-Guided Expression from Sequence to Purified Material
Key Features:
- AI Host Prediction — Graph neural networks analyze target sequence, domain architecture, and post-translational modification requirements to rank E. coli, yeast, insect, and mammalian expression probability.
- Codon Optimization — Host-specific codon adaptation with mRNA secondary structure minimization, eliminating rare codon bottlenecks and improving translation efficiency.
- ML Fermentation Optimization — LSTM models predict optimal fed-batch profiles, reducing trial-and-error runs and improving yields by 70–100%.
What We Offer: For virtual biotechs without molecular biology teams, AI prediction eliminates weeks of host screening. For pharma, codon-optimized constructs feed directly into Crystal Grade Protein Preparation with full traceability.
Explore Gene-to-Protein Production →Crystal Grade Protein Preparation
From Crude Lysate to Diffraction-Ready Quality
Key Features:
- Multi-Step Chromatography — IMAC, ion exchange, and size-exclusion chromatography with AI-guided buffer optimization for monodispersity and thermal stability.
- Real-Time Quality Gates — NanoDSF thermal profiling, DLS polydispersity index, and SEC-MALS oligomeric state verification at every purification stage.
- Structural Readiness Assessment — Direct handoff to AI-Assisted X-ray Crystallography Services, AI-Enhanced Cryo-EM Services, or AI-Enhanced NMR Spectroscopy Services.
What We Offer: For Fragment-based Screening (FBS) programs, crystal-grade protein with Tm >50°C and PDI <1.1 ensures reliable SPR and STD-NMR validation.
Explore Crystal Grade Protein Preparation →Technology Platform
Integrated Production Infrastructure: AI Design + Fermentation + Purification, Zero Handoffs
Traditional protein production splits gene design, expression, and purification across separate vendors — losing critical quality context at every handoff. Our platform unifies all three stages under one project team, with AI predictions informing fermentation conditions and real-time QC data feeding back into construct optimization.
Computational Platform — Dry Lab
| Capability | Details |
|---|---|
| AI/ML Expression Engine | GNN host prediction, LSTM fermentation optimization, codon adaptation with mRNA structure prediction |
| Structure-Guided Construct Design | AlphaFold domain boundary analysis; Homology Modeling for complex stoichiometry |
| PERISCOPE-Opt Integration | Open-access ML webserver for E. coli periplasmic expression yield prediction |
Experimental Production Platform — Wet Lab
| Capability | Details |
|---|---|
| Multi-System Expression | E. coli (auto-induction, fed-batch), yeast (Pichia, Saccharomyces), insect (Sf9, Hi5 baculovirus), mammalian (HEK293, CHO) |
| Purification Chromatography | AKTA Pure/Avant with IMAC, IEX, SEC, HIC, and affinity columns |
| Quality Validation | Prometheus NT.Plex NanoDSF, Wyatt SEC-MALS, Zetasizer DLS |
Tecniplast ZEBTEC Multi-Rack System
Bio-Rad CFX384 Touch
Tecan Fluent Liquid Handler
Sartorius Biostat B-Plus 5L
Platform Edge: The ability to predict expression failure before culture inoculation — then pivot to an alternative host without restarting the project clock — protects your timeline and budget.
Closed-Loop Discovery Engine
When Prediction Meets Fermentation Truth
Static construct designs assume ideal expression conditions. In reality, protein stability, solubility, and yield depend on dynamic interactions between sequence, host, and fermentation parameters. Our platform feeds every experimental result back into the AI models — so each production campaign improves the next.
AI Expression Design
AlphaFold-guided construct boundaries and host prediction inform cloning strategy
→ Feeds into Wet-lab
ML Fermentation Optimization
LSTM-predicted conditions guide fed-batch profiles, reducing empirical screening
→ Feeds into Purification
Industrial Value:
For Biotechs
Your first protein's quality data trains the models for your second. Structural validation from Phase 0 targets becomes training data for Phase 1 — a compounding learning partnership.
For Pharma
Every AI prediction is linked to an experimental outcome with project ID, timestamp, and model version — fully audit-ready for regulatory submissions and internal portfolio reviews.
Project Management & Execution
Project Workflow
A standardized, milestone-driven execution system. From sequence intake to crystal-grade protein delivery.
01 Strategy
- Target sequence review, druggability assessment, AI host prediction
Deliverable: Expression strategy report
02 AI Design
- Codon optimization, construct design, AlphaFold domain boundary analysis
Deliverable: Sequence-verified constructs
03 Expression
- Parallel expression in 2–4 host systems, small-scale induction, yield assessment
Deliverable: Expression screen results
04 Purification
- Scale-up in predicted optimal host, ML-guided fermentation, multi-step chromatography
Deliverable: Purified protein with yield documentation
05 Validation
Deliverable: Quality certificate + readiness assessment
06 Deliverables
- Quality certificate, structural readiness report, handoff to Crystallography or Cryo-EM
Deliverable: Final report + data package + transition plan
Sample Requirements
| Sample Type | Specification |
|---|---|
| Target Sequence | FASTA format; UniProt ID; domain boundaries; post-translational modification requirements |
| Expression Priority | Soluble intracellular, secreted, membrane-bound, or complex assembly |
| Structural Endpoint | X-ray, Cryo-EM, NMR, or biophysical assay |
| Quantity Needed | Milligram to gram scale; single-batch or recurring production |
Standard Deliverables
Upon project completion, clients receive comprehensive experimental reports including:
- AI-optimized expression strategy report with host and codon recommendations
- Sequence-verified expression constructs
- Purified protein with yield, purity, and concentration documentation
- NanoDSF thermal stability profile with Tm and onset temperatures
- DLS polydispersity index and SEC-MALS oligomeric state analysis
- Structural readiness assessment and direct handoff to downstream services
Our technical team responds within 24 hours. All inquiries protected under NDA.
Frequently Asked Questions
Case Study
Case #1: Custom Protein Production and Nanodisc Assembly
Goal: Produce the F2 receptor membrane protein and assemble it in nanodiscs for enhanced stability and solubility — enabling downstream SPR binding assays and co-crystallization.
Key Data:
- Expression system: Cell-free system with cDNA optimized for pET23b vector (BamHI/XhoI), His- and FLAG-tagged at C-terminus
- Nanodisc optimization: Seven concentrations tested (10–120 µM); 120 µM achieved highest F2 yield in supernatant
- Purification: Ni²⁺-NTA affinity chromatography with stepwise imidazole elution (20–500 mM)
- Quality: SDS-PAGE confirmed F2 receptor (42.9 kDa) and scaffold protein (26 kDa); Western blot (anti-FLAG) verified identity
Why it matters: Membrane proteins require native lipid environments for structural integrity. This case demonstrates our end-to-end capability — from cell-free expression optimization to nanodisc assembly to affinity purification — delivering a stable, detergent-free membrane protein complex ready for biophysical characterization or structural determination. For seed-stage biotechs, this eliminates the need to coordinate expression, lipid preparation, and purification vendors. For pharma, the optimized 120 µM nanodisc condition provides a reproducible platform for GPCR and ion channel programs.
Figure 1. SDS-PAGE of F2-nanodisc complex supernatant across nanodisc concentration gradient (10–120 µM). Red arrow: F2 (42.9 kDa); yellow arrow: scaffold protein (26 kDa).
Figure 2. SDS-PAGE of F2-nanodisc complex precipitation across nanodisc concentration gradient.
Figure 3. Western blot (anti-FLAG) confirming F2 receptor identity in purified complex. Red arrow: F2 (42.9 kDa).
