High-throughput screening (HTS) remains the key approach for finding hits and leads in the field of drug discovery. As the number of compounds available for screening has increased dramatically, industrialization and automation of the process have improved the ability to deal with larger numbers of compounds. Creative Biostructure provides high-quality custom MagHelix™ HTS assay development services for the pharmaceutical and biotechnological industries. We can start from a specific target/cell line or assist an ongoing lead discovery programs based on the requirements of our customers.
In general, the quality of an assay is defined by the robustness and reproducibility of this signal in the absence of any test compounds or in the presence of inactive compounds. The quality of the HTS is then defined by the behavior of this assay system when screened against a series of compounds. Assay development for HTS can be roughly characterized as cell-free or cell-based in nature. The aim of assay development is to transfer the assay parameters to a new format, thereby enhancing dynamic range, sensitivity, signal intensity, or stability of the assay.
Figure 1. Assay development cycle.
Creative Biostructure is capable of developing and executing high-throughput biochemical/cell-based screening assays for a variety of targets such as receptors, enzymes, and other proteins. We support the development of a wide range of detection methods, including but not limited to, standard plate reader assays, radioactivity, flow cytometry, electrochemiluminescence, fluorescence, mass spectrometry, imaging, etc. Utilizing our technical expertise in cell biology, molecular biology, and analytical chemistry, Creative Biostructure can also work with customers to design and develop novel assays. Our seasoned scientists are able to transfer the assays into HTS approaches. All assay procedures, specifications, and results can be precisely documented for our customers.
As a contract service provider in the field of drug discovery, Creative Biostructure offers HTS assay development services for the biotechnological and pharmaceutical industries. Our professional scientists are confident to provide high-quality services to global customers at competitive prices.