Gene-to-Protein Production
AI-Guided Construct Design 
High-Throughput Expression 
Scalable Purification Seed-stage biotechs lack protein infrastructure; pharma teams battling membrane proteins need native-conformation supply. We deliver AI-optimized constructs, parallel expression screening, and crystallization-grade purification — closing the gap between sequence and structure.
Why Gene-to-Protein Production Is the Critical Foundation
Structure-based drug design without reliable protein supply is synthesis without substrate. Seed-stage biotechs lack expression infrastructure; big-pharma teams need proven membrane-protein and nanodisc expertise. Our AI-Enhanced Structural Analysis platform integrates AlphaFold Protein Structure Prediction-guided construct design with parallel screening and scalable purification, delivering assay-ready proteins directly into AI-Assisted X-ray Crystallography Services, AI-Enhanced Cryo-EM Services, or Hit Biophysical Characterization workflows.
What Sets the Platform Apart
AI-Guided Construct Design
AlphaFold Protein Structure Prediction pLDDT maps guide truncation boundaries and solubility-tag selection, cutting trial-and-error by 30–50%.
Multi-System Parallel Screening
E. coli, yeast, insect, and mammalian cells screened in parallel; optimal expression conditions locked within 1 week.
Membrane Protein & Nanodisc Expertise
Specialized detergent screening, lipid reconstitution, and MSP nanodisc assembly preserve native membrane-protein conformations for Membrane Protein & Lipid MD Simulation.
Technology Suite
AI-Assisted Construct Engineering & Parallel Expression Screening
AI-optimized construct design and multi-host parallel screening for rapid expression condition identification.
Key Features:
- AlphaFold-Guided Truncation Mapping — pLDDT/pAE confidence scores from AlphaFold Protein Structure Prediction define soluble domain boundaries and flag disordered termini for removal, reducing expression failure rates.
- Host-Optimized Codon Engineering — Deep-learning codon optimization tailored to each expression host (E. coli, Pichia, Sf9, HEK293) improves yield and solubility.
- 96-Well Parallel Expression Screening — Four-host, multi-vector small-scale induction with SDS-PAGE solubility readout; 96 targets assessed in 1 week.
- ML Tag Recommendation — Proprietary classifiers trained on 500+ in-house productions recommend fusion partners (MBP, GST, SUMO, Fc) for optimal folding.
Ideal For: Virtual biotechs without wet-lab infrastructure; difficult targets (kinases, GPCRs, ion channels); programs requiring rapid protein supply for Molecular Docking Services or Virtual Screening Services.
What We Offer: A fully outsourced gene-to-protein pipeline. You provide a sequence; we deliver expression reports, purified protein, and a validated COA. AI-guided design compresses traditional months-long construct optimization into weeks, while parallel screening ensures no expression host is overlooked.
Scalable Purification & Quality Validation
Automated chromatography scale-up with rigorous homogeneity, activity, and stability assessment.
Key Features:
- AKTA Pure Chromatography Scale-Up — Automated affinity capture (IMAC, Strep-Tactin) followed by IEX and SEC polishing; milligram to gram scale.
- SEC-HPLC & DLS Profiling — Rigorous homogeneity and aggregation-state assessment before release.
- Membrane Protein Reconstitution — Detergent exchange, liposome insertion, or MSP nanodisc assembly for native lipid-bilayer environments.
- Activity & Thermal Validation — BLI/SPR binding confirmation and NanoDSF thermal stability profiling to predict crystallizability.
Ideal For: Crystal Grade Protein Preparation for AI-Assisted X-ray Crystallography Services; biophysical screening campaigns; antibody-antigen complex assembly for AI for Antibody & Biologics.
What We Offer: Every batch ships with a COA (concentration, purity, endotoxin, activity, thermal stability). Membrane proteins are delivered in nanodiscs or proteoliposomes with functional validation data, ready for MagHelix™ Co-crystallization and Soaking or Single Particle Analysis (SPA).
Platform Instrumentation
Core Instruments
| Instrument | Capability |
|---|---|
| Cytiva ÄKTA pure / ÄKTA avant | Automated protein purification from analytical to preparative scale |
| Thermo Fisher Vanquish Neo UHPLC | High-resolution SEC-HPLC for homogeneity and purity profiling |
| Formulatrix Rock Imager 1000 | High-throughput crystallization screening and imaging |
| Malvern Zetasizer Nano | DLS particle size and aggregation-state analysis |
| Sartorius Octet SF3 | BLI activity and binding validation |
| NanoTemper Prometheus NT.48 | NanoDSF thermal stability for crystallizability prediction |
| Thermo Fisher Krios G4 | Downstream Cryo-EM structural validation |
| NVIDIA DGX A100 | AlphaFold inference and protein-stability ML model training |
Standardized Workflow
Project Workflow
A milestone-driven system from sequence to validated protein.
01 Target Review
- Sequence analysis and AlphaFold Protein Structure Prediction assessment
- Truncation design and codon optimization
- Expression system selection
02 AI-Guided Construct Design
- Gene synthesis and vector construction
- Four-host small-scale expression screening
- Solubility and yield assessment
03 Expression & Purification
- Scale-up culture and induction optimization
- Affinity capture and polishing (IEX/SEC)
04 Quality Validation
- SEC-HPLC purity check
- DLS aggregation analysis
- NanoDSF thermal stability
- BLI activity validation
05 Downstream Handoff
- Protein handoff to AI-Assisted X-ray Crystallography Services or AI-Enhanced Cryo-Electron Microscopy (Cryo-EM) Services
- Co-crystal soaking or SPA grid preparation
Sample Requirements
- Target Sequence: Amino acid sequence (FASTA) or UniProt ID; membrane proteins require topology annotation
- Expression System Preference: E. coli, yeast, insect, or mammalian (if known)
- Post-Translational Modifications: Glycosylation, phosphorylation, or disulfide-bond requirements
- Purity & Quantity: Screening grade (>90%) or crystallography grade (>95%); mg to g scale
- Downstream Application: AI-Assisted X-ray Crystallography Services, AI-Enhanced Cryo-EM Services, SPR/BLI, or Molecular Docking Services
Standard Deliverables
- Purified protein with COA (concentration, purity, endotoxin, activity, thermal stability)
- Expression and purification technical report with chromatograms and gel images
- AI-assisted construct design rationale and AlphaFold Protein Structure Prediction analysis summary
- Stability assessment data (NanoDSF, DLS aggregation profiles)
- Electronic data package (sequences, plasmid maps, raw analytical data)
Frequently Asked Questions
Case Study
Case #1: Isolation and Purification of Native R1 Complex
Goal: Isolate and purify the native R1 protein complex (four subunits: A, B, C, D) from tissue samples for downstream structural and functional studies.
Key Data:
- Source: Mouse tissue samples (kidney, liver, skin, spleen)
- Detection: Western blot identified B subunit (130 kDa) specifically in spleen RIPA lysate
- Purification: Anti-B antibody-conjugated NHS-activated agarose affinity chromatography
- Validation: SDS-PAGE and Western blot confirmed intact R1 complex purification with high specificity
Why it matters: Multi-subunit native complexes often resist recombinant expression. This case demonstrates our expertise in antibody-conjugated affinity chromatography for endogenous complex isolation — a critical capability when recombinant systems fail. For biotechs targeting native assemblies without expression clones, this provides a viable path to pure material. For pharma, the customized conjugation and purification workflow delivers structurally stable complexes for Cryo-EM or biophysical assay validation.
Figure 1. Western blot analysis of mouse B protein across tissue samples. Red arrow indicates target band (130 kDa) in spleen RIPA lysate (Lane 11).
Figure 2. SDS-PAGE confirming anti-B antibody conjugation to NHS-activated agarose beads. Lane 1: pre-conjugation; Lane 2: post-conjugation.
Figure 3. SDS-PAGE (left) and Western blot (right) of purified R1 complex. FT: flow-through; W1-W2: washes; E1-E3: elutions. Red arrow indicates target protein.
Our technical team responds within 24 hours. All inquiries protected under NDA.
