MagHelix™ Toxicity Assays Using Zebrafish

Inquiry

Creative Biostructure is a biotechnology company offering drug discovery contract services to pharma, biotech, chemical companies, and academic institutes. Based on our zebrafish screening platform, we can provide MagHelix™ toxicity assays using zebrafish. Zebrafish has been a prominent model vertebrate for investigating chemical toxicity. We aim at developing new and faster screen/bioassays using zebrafish as an animal model, as well as new technologies for optimizing the maintenance and breeding of zebrafish.

Figure 1. Visualization of the zebrafish embryo vasculature by gfp:fli1 expressing endothelial cells.

Our MagHelix™ Toxicity Assay Services include but are not limited to:

General Toxicity Assays
Acute Toxicity Assay	Following the OECD 236 Guideline (Fish Embryo Acute Toxicity Test), the test is to determine the acute or lethal toxicity of chemicals on embryonic stages of fish (Danio rerio).
Reproductive/Developmental Toxicity Assay	To understand the potential of a drug to induce birth defects in offspring; that is to detect toxicity to reproduction and/or male fertility for medicinal products.
Specific Toxicity Assays
Cardiotoxicity Assay	Integrating of fluorescent reporter genes into the zebrafish model allows visualization of specific tissues, such as the heart.
Hepatotoxicity Assay	Mechanisms that occur during the process of development have been characterized, which helps the study of signaling pathways that govern zebrafish liver function.
Neurotoxicity Assay	The zebrafish is used to fill the gaps between various in vitro and in vivo models currently being used to identify mechanisms of developmental neurotoxicity.
Ototoxicity Assay	Zebrafish lateral line has been proved as a powerful screening tool for the identification of potentially ototoxic drugs.
Multi-organ Toxicity Assay	High-throughput zebrafish screens are becoming an important tool in identifying compounds with potential three main (cardiac-, hepato- and neuro-) toxicity liabilities in the same animal.

Advantages of our MagHelix™ Toxicity Assay services:

Applied for preclinical drug assessment, and the toxicity assay results are the basis for the optimization of drug candidates, which is conducive to reducing the cost of research and development.
We have a professional zebrafish screening platform for the development of disease models and in vivo drug screening.
Flexible lead compound toxicity assessment services, we also have extensive experience in computational toxicity prediction and high-throughput in vitro ADME-Tox profiling.
For each project, our team of drug discovery experts will provide an appropriate solution and actively communicate with customers.
We adopt a strict confidentiality policy for project information and experimental data.

Creative Biostructure provides high-value services for any organization with a need for zebrafish model development or drug toxicity evaluation. We have the ability to provide highly efficient and customized toxicity assays to meet your requirements.

References

Panzica-Kelly J M.; et al. Zebrafish embryo developmental toxicology assay. Developmental Toxicology. Humana Press, Totowa, NJ. 2012: 25-50.
TG236 O. OECD guidelines for the testing of chemicals section 2: Effects on biotic systems test No 236: fish embryo acute toxicity (FET) test. Organization for Economic Cooperation and Development. Paris, France, 2013.
Kiper K G, Freeman J L. Zebrafish as a tool to assess developmental neurotoxicity. Cell Culture Techniques. Humana, New York, NY, 2019: 169-193.